Coding

Part:BBa_K5233013

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-28)


MHETase(PelB), a kind of tannase family protein that can degrade MHET

MHETase is a kind of tannase family protein that can degrade MHET. We use it to degrade MHET, the intermediate products, to the TPA. Signal peptide PelB is used here to make the engineering bacteria secrete the MHETase outside the cell


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1478
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1478
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1478
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1478
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 451
    Illegal AgeI site found at 1101
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None